最近的nature protocolS（转自dxy)
最近的nature protocolS(超赞,转自dxy)
谢谢petersonswh
一些最近的NATURE PROTOCAL 文章。这些资料包含面较广，从植物到线虫到哺乳动物细胞培养的方法都有。文章大多来自较著名的实验室，结果很漂亮，方法也很实用，衷心希望能对大家的实验有所启发，我也会在今后收集更多的此类方法性文章和大家分享。为了方便大家阅读，我已将每篇pfd文件做了标注，可以直接找到你需要的篇名。
A protocol for differential display of mRNA expression using either fluorescent or radioactive labeling - pp457 - 470
Heterotopic vascularized murine cardiac transplantation to study graft arteriopathy - pp471 - 480
A protocol for isolation and culture of human umbilical vein endothelial cells - pp481 - 485
A simple, robust and semi-automated parasite egg isolation protocol - pp486 - 489
In vitro screening for molecules that affect virus capsid assembly (and other protein association reactions) - pp490 - 498
Acquisition and voxelwise analysis of multi-subject diffusion data with Tract-Based Spatial Statistics - pp499 - 503
The chemoinvasion assay: a method to assess tumor and endothelial cell invasion and its modulation - pp504 - 511
Immuno-spin trapping analyses of DNA radicals - pp512 - 522
Design and construction of targeted AAVP vectors for mammalian cell transduction - pp523 - 531
Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging - pp532 - 540
Chemically induced mouse models of intestinal inflammation - pp541 - 546
Freeze-fracture electron microscopy - pp547 - 576
Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes - pp577 - 587
Data analysis of assorted serum peptidome profiles - pp588 - 602
Sequencing complete mitochondrial and plastid genomes - pp603 - 614
Synthesis and oligonucleotide incorporation of fluorescent cytosine analogue tC: a promising nucleic acid probe - pp615 - 623
Parallel synthesis of peptide libraries using microwave irradiation - pp624 - 631
The use of the Ugi four-component condensation - pp632 - 639
Selenium modification of nucleic acids: preparation of phosphoroselenoate derivatives for crystallographic phasing of nucleic acid structures - pp640 - 646
Selenium modification of nucleic acids: preparation of oligonucleotides with incorporated 2'-SeMe-uridine for crystallographic phasing of nucleic acid structures - pp647 - 651
Purification of mitochondrial and plastid DNA - pp652 - 660
Non-invasive measurement of bioelectric currents with a vibrating probe - pp661 - 669
Mixed-culture assays for analyzing neuronal synapse formation - pp670 - 676
Pulsed-field gel electrophoresis - pp677 - 684
MetaNetwork: a computational protocol for the genetic study of metabolic networks - pp685 - 694
Uptake and release protocol for assessing membrane binding and permeation by way of isothermal titration calorimetry - pp695 - 704
A protocol describing pharynx counts and a review of other assays of apoptotic cell death in the nematode worm Caenorhabditis elegans - pp705 - 714
Determining the stoichiometry and interactions of macromolecular assemblies from mass spectrometry - pp715 - 726
Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox - pp727 - 738
Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis - pp739 - 752
A real-time method of imaging glucose uptake in single, living mammalian cells - pp753 - 762
Identification of host-induced pathogen genes by differential fluorescence induction reporter systems - pp770 - 777
Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry - pp778 - 791
A method for genetic modification of human embryonic stem cells using electroporation - pp792 - 796
In vivo imaging of subcutaneous structures using functional photoacoustic microscopy - pp797 - 804
A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood - pp805 - 810
Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain construction - pp811 - 821
Genetic manipulation of Aspergillus nidulans: heterokaryons and diploids for dominance, complementation and haploidization analyses - pp822 - 830
Protocol for the purification of proteins from biological extracts for identification by mass spectrometry - pp831 - 837
RNA stable-isotope probing - pp838 - 844
Practical and convenient synthesis of coumarins from phenols and propiolic acid esters - pp845 - 848
A combined ex/in vivo assay to detect effects of exogenously added factors in neural stem cells - pp849 - 859
DNA stable-isotope probing - pp860 - 866
Rapid measurement of total antioxidant capacity in plants - pp867 - 870
Measurement of reduced, oxidized and total ascorbate content in plants - pp871 - 874
Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent - pp875 - 877
Total internal reflection with fluorescence correlation spectroscopy - pp878 - 890
Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes - pp891 - 903
Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances - pp904 - 923
Gene splicing and mutagenesis by PCR-driven overlap extension - pp924 - 932
Chemical derivatization of histones for facilitated analysis by mass spectrometry - pp933 - 938
Preparation of cultured myofibers from larval salamander limbs for cellular plasticity studies - pp939 - 947
Agrobacterium rhizogenes-mediated transformation of soybean to study root biology - pp948 - 952
Locating proteins in the cell using TargetP, SignalP and related tools - pp953 - 971
An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with 99mTc at room temperature - pp972 - 978
A protocol for conducting 7-day daily renewal tests with Lemna gibba - pp979 - 987
Mapping networks of physical interactions between genomic elements using 5C technology - pp988 - 1002
Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies - pp1003 - 1012
Synthesis, purification and sample experiment for fluorescent pteridine-containing DNA: tools for studying DNA interactive systems - pp1013 - 1021
Immobilization of enzymes on heterofunctional epoxy supports - pp1022 - 1033
Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells - pp1034 - 1043
Isolation and culture of rat and mouse oligodendrocyte precursor cells - pp1044 - 1051
Synthesis of oligo-RNAs with photocaged adenosine 2'-hydroxyls - pp1052 - 1058
Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis - pp1059 - 1069
Identification and insertion of 3-carbon bridges in protein disulfide bonds: a computational approach - pp1070 - 1083
Cytokinesis-block micronucleus cytome assay - pp1084 - 1104
Cis-regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution - pp1105 - 1116
In vivo imaging of vesicle motion and release at the Drosophila neuromuscular junction - pp1117 - 1125
Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli - pp1126 - 1133
Development and optimization of high-throughput in vitro protein phosphatase screening assays - pp1134 - 1144
Tandem affinity purification of functional TAP-tagged proteins from human cells - pp1145 - 1151
Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry - pp1152 - 1165
A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) - pp1166 - 1172
Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM) - pp1173 - 1179
Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells - pp1180 - 1184
Imaging mass spectrometry at cellular length scales - pp1185 - 1196
Selective proliferation of rat hepatocyte progenitor cells in serum-free culture - pp1197 - 1205
Pronuclear injection for the production of transgenic mice - pp1206 - 1215
Stripe assay to examine axonal guidance and cell migration - pp1216 - 1224
Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation
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